Scott M. Auerbach and Wise Young, ``Total phosphate determination
in brain tissue: A method for regional determination ot total phosphate
in rat brain,'' CNS Trauma 4, 53-61 (1987).
Abstract
A quantitative microassay method is described for brain tissue
phosphates. Based on molybdate colorimetric measurements of inorganic
phosphate in tissue solutions prepared by acid digestion and high
temperature ashing, the method includes the use of calcium to precipitate
inorganic phosphate in acid-digested tissues and a correction for
contaminants released from porcelain crucibles during the ashing
procedure. This method was used to measure the total tissue phosphate
concentrations in regional microsamples of rat brain. Averaged
values derived from these regional measurements were 100.1 +/-
10.2 micromolar/gram wet tissue weight, corresponding closely to whole
brain tissue phosphate values reported in the literature. Phosphate
concentrations were remarkably uniform in different areas of the cortex
and basal ganglia.
Prof SM Auerbach
28 August 2000